To determine the in vitro efficacy of glucoprotamin for the disinfection of instruments.
Prospective observational study.
University women's hospital.
Instruments were immersed in saline solution after use, and glucoprotamin was added to a concentration of 1.5% before soaking for 60 minutes. Biocidal activity was determined by the difference in colony-forming units (CFU) on instruments before and after disinfection.
One hundred thirty-seven instruments were collected during 10 days and exposed to a 1.5% dilution of glucoprotamin without prior washing. Bioburden before disinfection ranged from 2 x 10(5) to 7.1 x 10(7) CFU per instrument. Average bacterial killing was 5.98 log10 CFU +/- 0.48 under aerobic conditions and 6.75 log10 CFU +/- 0.54 under anaerobic conditions, despite the presence of large amounts of proteins on instruments that were frequently bloody. No vegetative bacteria were isolated in any sample after disinfection.
This clinical study confirmed excellent in vitro efficacy of glucoprotamin without prior removal of proteins and debris.
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